Lactic acid Adsorbent and method for removing Lactic acid
Reduces adverse effects on cell and microbial growth while increasing the efficiency of lactic acid removal
Overview
In recent years, there has been a demand for mass culture of cells in fields such as pharmaceutical manufacturing. In order to grow cells stably in culture, lactic acid (a cellular waste product that adversely affects cells) that accumulates in the culture medium must be removed. Until now, lactic acid was removed from the culture medium using the principle of dialysis. However, in order to remove lactic acid sufficiently, the volume of the component preparation liquid tank was set to be more than 10 times the volume of the cell culture tank, so there was a problem that the required volume of liquid was enormous and costly.
The present invention has made it possible to provide a new lactic acid removal technology that reduces the negative effects of lactic acid and lactic acid removal treatment on the growth of cells and microorganisms while increasing the lactic acid removal efficiency. An embodiment of the present invention is a lactic acid adsorbent, characterized in that it comprises a layered double hydroxide having anions and water molecules retained between layers of a plurality of metal hydroxide layers. When the present invention comes into contact with a solution containing lactic acid, lactic acid in the solution is adsorbed. Thus, the removal efficiency of lactic acid can be enhanced.
It was confirmed that the adsorption rate of lactic acid was high, but the adsorption rate of glucose was low, and that the adsorption rate of lactic acid was very low
Product Application
・Application to culture medium regeneration technology in mass culture of cells, etc.
Related Works
[1] Kameda T, et al. Colloids and Surfaces A: Physicochemical and Engineering Aspects 612 (2021) 125975.
IP Data
IP No. ： JP7191341, JP7264397, JP7236120
Joint applicant ：Nikkiso Co., Ltd.
Inventor ： YOSHIOKA Toshiaki, KAMEDA Tomohito
keyword ： Lactic acid absorbent, LDH