Strong promoter for marine invertebrates

OsHV-1 promoter for gene delivery and a variety of applications in bivalve molluscs.

Overview

　Understanding gene functions in marine invertebrates has been limited, largely due to the lack of suitable assay systems.　

　The inventors discovered a strong viral promoter derived from a bivalve-infectious virus, ostreid herpesvirus-1 (OsHV-1).

　This promoter showed 24.7-fold and 16.1-fold higher activity than the cytomegalovirus immediate early (CMV IE) promoter and the endogenous EF1α promoter, the two most commonly used promoters in bivalves so far (Fig.1).

　GFP assays showed that the OsHV-1 promoter is active not only in scallop cells (Fig.2) but also in HEK293 cells (Fig.3) and zebrafish embryos.

The OsHV-1 promoter activities

Product Application

・Modification of taste and nutritional content　
・Provision of water purification function
・Development of genetic sterilization technology
・Production of superior varieties
・Production of functional proteins

Related Works

[1] PNAS 2022 Vol. 119 No. 45　e2209910119　

IP Data

IP No.　 ：PCT/JP2022/030912　　
Inventor ：YOON Jeongwoong, NAGASAWA Kazue, OSADA Makoto, YOKOI Hayato
keyword ：transformation, geneticengineering, fisheries, bivalves