Fermentation of fully-dispersed filamentous fungi mutant

Capable of several-fold production of the desired product!

Overview

　Filamentous fungi (e.g. Aspergillus oryzae) are well-used for production of useful proteins and small molecules in industry. However, it is well-known that hyphal aggregation during the liquid cultivation often prevents from growing with high density, resulting in low productivity. This invention discloses a mutant fungus, in which α-1,3-glucan synthase (AGS) genes plus the genes on galactosaminogalactan (GAG) cluster are deficient.　
　AGS deficient fungi (AGΔ) showed a highly dispersed property [1]. By inducing further deficiency in genes on GAG cluster, the mutant (AGΔ-GAGΔ) shows a fully dispersed state in liquid culture. Further, this phenotype contributes to well aerobic conditions during cultivation, resulting in increasing growth of cells and substance production thereby.

Distinct morphology / mixing property

Increased biomass/productivity

Product Application

・Production of functional proteins/peptides like enzymes　　
・Production of small molecules with biological activities such as amino acids, antibiotics, etc..
・Application of increased fungi biomass to meat alternative

Related Works

[1] Miyazawa et al (2019) Front Microbiol 10:2090
[2] Miyazawa et al (2020) Fungal Biol Biotechnol 7(10) 1-13
[3] Ichikawa et al (2022) J Biosci Bioeng 133(1):39-45

IP Data

IP No.　 ： JP6647653B2、US11021725B2, etc..　
Inventor ： ABE Keietsu, YOSHIMI Akira, MIYAZAWA Ken, TABATA Fuka, GOMI Katsuya
keyword ： fermentation, filamentousfungimutant, meatalternative